SD Polymerase 10 U/µl is a novel artificial thermostable polymerase with strong Strand Displacement, but lacking 5′-3′ exonuclease activity. It possesses heat stability up to 93 °C. With SD Polymerase possibilities of Isothermal Amplification and regular PCR can be combined. The efficiency of Isothermal Amplification can be raised by initial denaturation steps to increase the accessibility of single strands. SD Polymerase is efficient for high GC-content and difficult targets. Long Range PCR can be performed with target size up to 30 kb. SD Polymerase can be used in various strand displacement reactions like LAMP or WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions.

SD Polymerase 50 U/µl is a novel artificial thermostable polymerase with strong Strand Displacement, but lacking 5′-3′ exonuclease activity. It possesses heat stability up to 93 °C. With SD Polymerase possibilities of Isothermal Amplification and regular PCR can be combined. The efficiency of Isothermal Amplification can be raised by initial denaturation steps to increase the accessibility of single strands. SD Polymerase is efficient for high GC-content and difficult targets. Long Range PCR can be performed with target size up to 30 kb. SD Polymerase can be used in various strand displacement reactions like LAMP or WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions.

SD Polymerase Hotstart 10 U/µl is a novel artificial thermostable polymerase with strong Strand Displacement, but lacking 5′-3′ exonuclease activity. It possesses heat stability up to 93 °C. With SD Polymerase Hotstart possibilities of Isothermal Amplification and regular PCR can be combined. The efficiency of Isothermal Amplification can be raised by initial denaturation steps to increase the accessibility of single strands. SD Polymerase Hotstart is efficient for high GC-content and difficult targets. Long Range PCR can be performed with target size up to 30 kb. The optimal blocking by antibody inactivates the enzyme until initial heating. SD Polymerase Hotstart can be used in various strand displacement reactions like WGA, Isothermal Amplification, PCDR, library generation and standard PCR reactions. For LAMP assays we strongly recommend SD Polymerase 50 U/µl without antibody.

SD Polymerase is also available without antibody for applications where Hotstart is not needed and as high concentrated variant with 50 U/µl for large scale applications.

Product                      Cat.No.       Packsize  

SD Polymerase, 10 u / µl                 108702        200 U

SD Polymerase, 10 u / µl                 108710        1000 U

SD Polymerase, 50 u / µl                 108800        1000 U

SD Polymerase, 50 u / µl                 108850        5000 U

SD Polymerase Hotstart, 10 u / µl           108902        200 U

SD Polymerase Hotstart, 10 u / µl           108910        1000 U